| PRODUCT NAME: | AccuBind Myoglobin |
| INTENDED USE: | The quantitative determination of circulating myoglobin concentration in human serum by a Microplate Enzyme Immunoassay. |
| PRODUCT OVERVIEW: | Myoglobin is one of the most important myocardial markers used in ruling out acute myocardial infarction (AMI) within 2 hours of admission because of chest pains. AMI disrupts cardiac cell membranes, releasing intracellular cardiac proteins into the vascular system. Myoglobin, being the smallest of these markers, diffuses rapidly throughout the vascular system and provides the earliest indication of AMI. Myoglobin levels rise between 0.5-2.0 hours after the onset of chest pain and peak within 5-12 hours. The kidneys rapidly eliminate myoglobin from the system, restoring normal circulating concentrations within 16-36 hours. Since the protein rapidly clears from the system, myoglobin concentrations can reliably indicate reinfarction. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Enzyme Immunoassay, Colorimetric |
| Principle: | Sandwich Assay, Streptavidin-Coated Plate |
| Calibrators: | 0, 10, 25, 50, 150, 400 ng/ml |
| Sample type: | Serum |
| Sample volume: | 25 µl
|
| Measuring range: | 0.178 - 400 ng/ml |
| Reading: | Absorbance at 450 nm |
| Total time: | 30 minutes
|
| Storage: | 2 - 8ºC
|
| Kit Contents: | Myoglobin Calibrators
Myoglobin Enzyme Conjugate
Streptavidin Plate
Wash Concentrate
Substrate A
Substrate B
Stop Solution
|