PRODUCT NAME: | AccuBind Alpha-Fetoprotein (AFP) |
INTENDED USE: | The quantitative determination of Alpha-Fetoprotein (AFP) concentration in human serum by a Microplate Enzyme Immunoassay. |
PRODUCT OVERVIEW: | Alpha-Fetoprotein (AFP) is a glycoprotein normally produced during fetal development by the hepatocytes, yolk sac and to a lesser extent by the gastrointestinal tract. Elevated levels of AFP are found in patients with primary heptatoma and yolk sac-derived germ tumors. AFP is the most useful marker for the diagnosis and management of hepatocellular carcinoma. AFP is also elevated in pregnant women. Presence of abnormally high AFP concentrations in pregnant women provides a risk marker for Down syndrome. |
TECHNICAL SPECIFICATIONS: |
| Method: | Enzyme Immunoassay, Colorimetric |
Principle: | Sandwich Assay, Streptavidin-Coated Plate |
Calibrators: | 0, 5, 25, 50, 250, 500 ng/ml (IRP 72/225) |
Sample type: | Serum |
Sample volume: | 25 µl |
Measuring range: | 1.0 - 500 ng/ml |
Reading: | Absorbance at 450 nm |
Total time: | 75 minutes |
Storage: | 2 - 8ºC |
Kit Contents: | AFP Calibrators Anti-AFP Enzyme Reagent Streptavidin Coated Plate Wash Concentrate Substrate A Substrate B Stop Solution |