| PRODUCT NAME: | ANAscreen |
| INTENDED USE: | ANAscreen is an ELISA test system for the qualitative measurement of IgG antibodies against nuclear antigens, SS-A 52, SS-A 60, SS-B, RNP-70, Sm, RNP/Sm, Scl-70, Centromere B, and Jo-1, in human serum or plasma. |
| PRODUCT OVERVIEW: | Antinuclear antibodies (ANA) are detected in patients with systemic autoimmune diseases such as systemic lupus erythematosus (SLE), Sjögren’s syndrome, Sharp syndrome, polymyositis/dermatomyositis, and progressive systemic scleroderma (PSS) with CREST syndrome. ANA thus partially act as makers for the individual connective tissue diseases. Each individual well of the ANAscreen test is coated with a mixture of nine human recombinant and native, highly purified antigens, allowing for sensitive detection of these autoantibodies. After a positive result with this test, further differentiation of the autoantibodies should be carried out with the ANAcombi test or the respective single antigen ELISA assays. |
TECHNICAL SPECIFICATIONS:
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| Method: | Indirect Enzyme Linked Immune Reaction |
| Capture: | Centromere B, histidyl-tRNA synthetase (Jo-1), RNP-70, RNP/Sm complex, Scl-70 protein (DNA topoisomerase I), Smith antigen (Sm), SS-A 52 (Ro 52), SS-A 60 (Ro 60), SS-B (La). |
| Sample type: | Serum and plasma |
| Sample volume: | 10 µl |
| Reading: | Absorbance at 450 nm |
| Cut-off: | Index > 1.2
|
| Total time: | 65 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Microplate
Controls: Positive & Negative
Enzyme Conjugate
TMB Substrate
Diluent
Stop Solution
Wash Concentrate
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