| PRODUCT NAME: | AccuBind Immunoglobulin E (IgE) |
| INTENDED USE: | The quantitative determination of Immunoglobulin E (IgE) concentration in human serum by a Microplate Enzyme Immunoassay. |
| PRODUCT OVERVIEW: | The measurement of immunoglobulin E (IgE) in serum is widely used in the diagnosis of allergic reactions and parasitic infections. Many allergies are caused by the immunoglobulins of subclass IgE acting as point of contact between the allergen and specialized cells. Before making any therapeutic determination it is important, however, to know whether the allergic reaction is IgE mediated or non-IgE mediated. Measurement of total IgE in serum sample, along with other supporting diagnostic information, can help to make that determination. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Enzyme Immunoassay, Colorimetric |
| Principle: | Sandwich Assay, Streptavidin-Coated Plate |
| Calibrators: | 0, 5, 25, 50, 150, 400 lU/ml (2nd IRP 75/502) |
| Sample type: | Serum |
| Sample volume: | 25 µl |
| Measuring range: | 0.125 - 400 IU/ml |
| Reading: | Absorbance at 450 nm |
| Total time: | 75 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Human Serum References
IgE Biotin Reagent
IgE Enzyme Reagent
Streptavidin Plate
Wash Concentrate
Substrate A
Substrate B
Stop Solution
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