PRODUCT NAME: | Anti-Insulin |
INTENDED USE: | Anti-Insulin is an ELISA test system for the quantitative measurement of IgG class autoantibodies against bovine, porcine and recombinant human insulin in human serum or plasma. |
PRODUCT OVERVIEW: | Anti-Insulin is an ELISA test for the quantitative determination of IgG antibodies against human, bovine and pig insulin in human serum or plasma. Type I diabetes mellitus is an autoimmune disease triggered by autoantibodies against pancreatic islet cells and against insulin itself. Autoantibodies against insulin can appear in the prediabetic phase of type I diabetes mellitus or once the condition manifests. These autoantibodies are found in 50 to 70 % of children with diabetes mellitus and 20 to 30 % of affected adults. In rare cases, insulin-dependent diabetes patients generate antibodies against exogenous insulin. These antibodies can give rise to insulin-resistance and should therefore be controlled regularly when monitoring insulin therapy. The wells of the Anti-Insulin ELISA are coated with a mixture of bovine, pig and recombinant human insulin. |
TECHNICAL SPECIFICATIONS: |
| Method: | Enzyme Linked Immune Reaction |
Capture: | Insulin (bovine, porcine, human recombinant) |
Calibrators: | 0, 6.3, 12.5, 25, 50, 100 U/mL |
Sample type: | Serum and plasma |
Sample volume: | 10 µl |
Measuring range: | 0 - 100 U/ml |
Reading: | Absorbance at 450 nm |
Cut-off: | 10 U/ml |
Total time: | 65 minutes |
Storage: | 2 - 8 ºC |
Kit Contents: | Microplate Calibrators: A, B, C, D, E, F Controls: Positive & Negative Enzyme Conjugate TMB Sbstrate Diluent Stop Solution Wash Concentrate |