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Free Prostate Specific Antigen (fPSA)

 
PRODUCT NAME: AccuBind Free Prostate Specific Antigen (fPSA)
INTENDED USE: The quantitative determination of Free Prostate Specific Antigen (fPSA) concentration in human serum by a Microplate Enzyme Immunoassay.
PRODUCT OVERVIEW: In human serum, PSA occurs in two forms: free PSA (f-PSA) and total PSA. The major form is a complex of PSA and α-antichymotrypsin (ACT). The fraction of f-PSA was shown to be substantially smaller in patients with untreated advanced prostate cancer (PCA) than in patients with benign prostate hyperplasia (BPH). Therefore combined measurements of f-PSA and t-PSA may lead to a better discrimination between BPH and PCA. Recent studies have shown that the f-PSA/t-PSA ratio is helpful in the differential diagnosis of BPH and PCA. 
TECHNICAL SPECIFICATIONS:

Method: Enzyme Immunoassay, Colorimetric
Principle: Sandwich Assay, Streptavidin-Coated Plate
Calibrators: 0, 0.5, 1, 2.5, 5, 10 ng/ml (1st IS 96/668)
Sample type: Serum
Sample volume: 50 µl
Measuring range: 0.052 - 10 ng/ml
Reading: Absorbance at 450 nm
Total time: 75 minutes
Storage: 2 - 8ºC
Kit Contents: fPSA Calibrators
fPSA Enzyme Conjugate
Streptavidin Coated Plate
Wash Concentrate
Substrate A
Substrate B
Stop Solution
*Please refer to the product Instruction-For-Use (IFU) for complete details.