| PRODUCT NAME: | 25-OH Vitamin D IR Elisa |
| INTENDED USE: | 25-OH Vitamin D IR ELISA is an ELISA based test system intended for the quantitative measurement of total concentration of 25-OH-Vitamin D2 and 25-OH-Vitamin D3 in human serum or plasma samples (EDTA plasma, heparin plasma, citrate plasma). |
| PRODUCT OVERVIEW: | Vitamin D3 (cholecalciferol) is produced in the skin under irradiation with UV-B rays from the sun. It is metabolized to 25-OH vitamin D3 in the liver. Vitamin D2 (ergocalciferol) is mostly produced synthetically and is used as a dietary supplement. It is also converted to its corresponding 25-OH derivative by the liver. Vitamin D regulates calcium levels in the blood and thus supports bone mineralization. Vitamin D deficiency leads to rickets in children and osteomalacia (bone softening) in adults. In people aged over 50 years, vitamin D levels are directly associated with bone density and a lack of vitamin D is a risk factor for osteoporosis. |
TECHNICAL SPECIFICATIONS:
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| Method: | Enzyme Immunoassay, Colorimetric |
| Principle: | Competitive, Antigen-Coated Plate |
| Control: | 2 levels of controls |
| Sample type: | Serum and plasma |
| Sample volume: | 80 µl
|
| Measuring range: | 5 - 120 ng/ml |
| Reading: | Absorbance at 450 nm |
| Total time: | 65 minutes
|
| Storage: | 2 - 8 ºC
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| Kit Contents: | Microwells
Calibrators (A - F)
2 Levels Of Control
Sample Diluent
Enzyme Conjugate
Releasing Reagent
Wash Buffer Concentrate
TMB Substrate
Stop Solution
Certificate of Analysis
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