PRODUCT NAME: | AccuBind Cortisol |
INTENDED USE: | The quantitative determination of cortisol concentration in human serum or plasma by a Microplate Enzyme Immunoassay. |
PRODUCT OVERVIEW: | Cortisol is a hormone produced by the adrenal glands. Physiologically effective in anti-inflammatory activity and blood pressure maintenance, cortisol is also involved in gluconeogenesis. Cortisol acts through specific intracellular receptors and has effects in numerous other physiologic systems, including immune function, glucose-counter regulation, vascular tone, substrate utilization and bone metabolism. Elevated cortisol levels have been identified in patients with Cushing's disease (ACTH hyper secretion) and in patients with adrenal tumors. Low cortisol levels are found in primary adrenal insufficiency and in ACTH deficiency. |
TECHNICAL SPECIFICATIONS: |
| Method: | Enzyme Immunoassay, Colorimetric |
Principle: | Competitive, Streptavidin-Coated Plate |
Calibrators: | 0, 1, 4, 10, 20, 50 µg/dl |
Sample type: | Serum or plasma |
Sample volume: | 25 µl |
Measuring range: | 0.366 - 50 µg/dl |
Reading: | Absorbance at 450 nm |
Total time: | 80 minutes |
Storage: | 2 - 8ºC |
Kit Contents: | Cortisol Calibrators Cortisol Enzyme Reagent Steroid Conjgate Buffer Cortisol Biotin Reagent Streptavidin Coated Plate Wash Concentrate Substrate A Substrate B Stop Solution |