| PRODUCT NAME: | Anti-Insulin |
| INTENDED USE: | Anti-Insulin is an ELISA test system for the quantitative measurement of IgG class autoantibodies against bovine, porcine and recombinant human insulin in human serum or plasma. |
| PRODUCT OVERVIEW: | Anti-Insulin is an ELISA test for the quantitative determination of IgG antibodies against human, bovine and pig insulin in human serum or plasma. Type I diabetes mellitus is an autoimmune disease triggered by autoantibodies against pancreatic islet cells and against insulin itself. Autoantibodies against insulin can appear in the prediabetic phase of type I diabetes mellitus or once the condition manifests. These autoantibodies are found in 50 to 70 % of children with diabetes mellitus and 20 to 30 % of affected adults. In rare cases, insulin-dependent diabetes patients generate antibodies against exogenous insulin. These antibodies can give rise to insulin-resistance and should therefore be controlled regularly when monitoring insulin therapy. The wells of the Anti-Insulin ELISA are coated with a mixture of bovine, pig and recombinant human insulin. |
TECHNICAL SPECIFICATIONS:
|
| Method: | Enzyme Linked Immune Reaction |
| Capture: | Insulin (bovine, porcine, human recombinant) |
| Calibrators: | 0, 6.3, 12.5, 25, 50, 100 U/mL |
| Sample type: | Serum and plasma |
| Sample volume: | 10 µl |
| Measuring range: | 0 - 100 U/ml |
| Reading: | Absorbance at 450 nm |
| Cut-off: | 10 U/ml |
| Total time: | 65 minutes
|
| Storage: | 2 - 8 ºC
|
| Kit Contents: | Microplate
Calibrators: A, B, C, D, E, F
Controls: Positive & Negative
Enzyme Conjugate
TMB Sbstrate
Diluent
Stop Solution
Wash Concentrate
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